The Experts Workshop on Finalization of the Practical Guide for Gender Analysis in Small-Scale Fisheries and Aquaculture in Southeast Asia was conducted from 10 to 11 July 2019 at SEAFDEC/Training Department (SEAFDEC/TD), Samut Prakan, Thailand. There were 40 participants (32 Females, 8 Males) from SEAFDEC Member Countries, renowned experts from regional partners, the SEAFDEC Gender Focal Persons (SGFP), SEAFDEC relevant staff and members of the Regional Fisheries Policy Network (RFPN) who were almost the same participants from the previous expert consultation workshop on gender last year August 2018. The experts workshop aimed to revise and finalize “the practical guide for gender analysis in small-scale fisheries and aquaculture in Southeast Asia” under categorized by thematic areas in accordance with the SSF Guidelines and developed methods and tools for gender analysis, including to provide gender-sensitive organization. Moreover, the ways forward and usage of this practical guide for application in the ASEAN Member States (AMSs) were discussed at the workshop.