The SEAFDEC Training Department (SEAFDEC/TD) organized “The Regional Training Course on Fish Handling Techniques Applicable to Various Fishing Operations in Southeast Asia” from 4 to 8 November 2019 at SEAFDEC/TD, Samut Prakan, Thailand. There were seven participants (6 males and 1 female) who relevant work experience on fish handling and can be a trainer in the future from the ASEAN Member States attended in this Training. The Training aimed to provide technical knowledge on fish handling techniques applicable to various fishing operations in Southeast Asia through imparted knowledge on the importance of hygiene, cleanliness and important key factors contributing to the reduction of post-harvest losses on-board. The subjects of the training included literature review, demonstration, and discussion of good practice for fish handling techniques on-board, fish storage, and ways to improve fresh quality at landing site using simple technique applied to various fisheries in the Southeast Asian region. Moreover, the appropriate and applicable methods for the reduction of post-harvest loss of coastal and offshore fishing vessels and maintaining the freshness of the catch thereby minimizing post-harvest losses were imparted. In the end, all participants expected to enhance knowledge and practical skills on the reduction of post-harvest losses which will help reinforce extension and promotion activities in their respective countries through improving awareness of hygiene and good practice of fish handling on-board.