The Online Regional Training Course of Fish Handling Techniques Onboard Fishing Vessels was organized by the SEAFDEC Training Department (SEAFDEC/TD) from 28 to 30 June 2022 via zoom platform. There were 14 participants (10 males and 4 females) from ASEAN Member States (AMSs) who have relevant work experience in fish handling and are capable to transfer the knowledge that gains from the training program for the improvement of fish handling in their respective area. Moreover, the training course aims to transfer appropriate knowledge of hygienic environmental/user-friendly fish handling tools through simple techniques that could be applied to maintain the quality of the catch products. In addition, views and ideas on practical skills and concepts related to the reduction of post-harvest losses in fisheries, including fish handling techniques, ice storage, preservation techniques, and transportation of fishery products were exchanged and discussed during the online training course. The training invited three resource persons who are experts in the mentioned subjects, namely Dr. Jirapa Hinsui from the Faculty of Fisheries, Kasetsart University including Dr. Walai Kleechaya and Mr. Pawared Inthuserdha from the Department of Fisheries to impart knowledge and share their experience in this course. This training is an activity under the project “Responsible Fishing Technology and Practice”.